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"This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology"--Provided by publisher

Includes bibliographical references at the end of each chapters and index.

Frontmatter -- Preface -- Foreword -- Contents -- List of contributing authors -- Part I: Basics -- 1 Brief history of fluorescence lifetime imaging / Kèonig, Karsten -- 2 The long journey to the laser and its use for nonlinear optics / Kaiser, Wolfgang -- 3 Advanced TCSPC-FLIM techniques / Becker, Wolfgang -- 4 Ultrafast lasers in biophotonics / Krueger, Arnd -- Part II: Modern nonlinear microscopy of live cells -- 5 STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities / Lanzano, Luca / Vicidomini, Giuseppe / Scipioni, Lorenzo / Castello, Marco / Diaspro, Alberto -- 6 Principles and applications of temporal-focusing wide-field two-photon microscopy / So, Peter T.C. / Choi, Heejin / Yew, Elijah / Rowlands, Christopher -- 7 FLIM-FRET microscopy / Alam, Shagufta Rehman / Melia, Meghan J.O / Wallrabe, Horst / Svindrych, Zdenek / Chandra, Dhyan / Joshi, Suchitra / Kapur, Jaideep / Periasamy, Ammasi -- 8 TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing / Rèuck, Angelika / Breymayer, Jasmin / Kalinina, Sviatlana -- 9 Laser tweezers are sources of two-photon effects / Kèonig, Karsten -- 10 Metabolic shifts in cell proliferation and differentiation / Shirmanova, Marina / Sergeeva, Tatiana / Druzhkova, Irina / Meleshina, Aleksandra / Lukina, Maria / Dudenkova, Varvara / Shcheslavskiy, Vladislav / Becker, Wolfgang / Belousov, Vsevolod / Mishina, Nataliya / Zagaynova, Elena -- 11 Femtosecond laser nanoprocessing / Kèonig, Karsten -- 12 Cryomultiphoton imaging / Breunig, Hans Georg / Kèonig, Karsten -- Part III: Nonlinear tissue imaging -- 13 Multiphoton Tomography (MPT) / Kèonig, Karsten -- 14 Clinical multimodal CARS imaging / Weinigel, Martin / Breunig, Hans Georg / Kèonig, Karsten -- 15 In vivo multiphoton microscopy of human skin / Balu, Mihaela / Kelly, Kristen M. / Harris, Ronald M. / Kèonig, Karsten / Zachary, Christopher B. / Tromberg, Bruce J. -- 16 Two-photon microscopy and fluorescence lifetime imaging of the cornea / Batista, Ana / Breunig, Hans Georg / Donitzky, Christoph / Kèonig, Karsten -- 17 Multiscale correlative imaging of the brain / Mascaro, Anna Letizia Allegra / Silvestri, Ludovico / Sacconi, Leonardo / Pavone, Francesco S. -- 18 Revealing interaction of dyes and nanomaterials by multiphoton imaging / Holmes, Amy / Thorling, Camilla / Liu, Xin / Liang, Xiaowen / Wang, Haolu / Breunig, Hans G. / Kèonig, Karsten / Studier, Hauke / Roberts, Michael S. -- 19 Multiphoton FLIM in cosmetic clinical research / Pena, Ana-Maria / Decenciáere, Etienne / Brizion, Sâebastien / Victorin, Steeve / Koudoro, Serge / Baldeweck, Thâeráese / Tancráede-Bohin, Emmanuelle -- 20 Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery / Kantelhardt, Sven R. -- 21 Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models / Uchugonova, Aisada / Hoffman, Robert M. -- 22 Bedside assessment of multiphoton tomography / Mess, Christian / Huck, Volker -- Index

English.

Added to collection customer.56279.3